Notes
Part Three
Prepare four additional diluted solutions. The most dilute solution you prepare
should be 1/10th of the concentration of the original solution. Measure the
absorbance values of all these solutions at the wavelength of maximum
absorbance.
The data table can be downloaded from Google Drive: Sample Data Tables.
Part Four
Measure the absorbance spectrum for the solution of unknown dye concentration.
Absorbance Value at λmax for Unknown Dye Solution:
This absorbance is likely higher than any of the solutions you have previously
measured. To determine the concentration of the unknown solution accurately,
we will need to dilute it and then measure the absorbance value of the diluted
solution. Pick the volumes for dilution based on what you found above.
•Volume of Unknown Dye Solution Used (mL):
•Volume of Volumetric Flask for Diluted Unknown Dye Solution
(mL):
•Absorbance Value at λmax for Diluted Unknown Dye Solution:
Sample Data Table for Measuring Absorbance Values for Dilutions | ||||
Solution Number | Volume of Original Dye Solution Used (mL) | Volume of Diluted Dye Solution (mL) | Molarity of Diluted Dye Solution (M) | Absorbance at wavelength of maximum absorbance |
1 | ||||
2 | ||||
3 | ||||
4 | ||||
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